Terminator

Part:BBa_K1510005:Design

Designed by: Yen-An Chang   Group: iGEM14_NYMU-Taipei   (2014-10-06)

B0012+B0012


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used back insert to assemble two terminator together. There's no point mutation in this construct. In order to fit iGEM standard part, there are EcoR1 and Xba1 cutting sites in the front and Spe1 and Pst1 cutting sites in the last.

NYMU yenanchang Electrophoresis of B0012+B0012.jpg

electrophoresis of B0012+B0012


Source

BBa_B0012+BBa_B0012(2014 iGEM kit)

References